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KMID : 0380020060210050394
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 5 p.394 ~ p.400
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The Development of Papain which is Extremely Stable to Negative Ionic Environment by Directed Molecular Evolution
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Kang Whan-Koo
Hwang Sun-Duk
Kim Hyoung-Sik
Jeung Jong-Sik
Lee Bheong-Uk
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Abstract
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When the papain, which is a sort of Cystein protease, is applied to the outer skin, it decomposes the protein which forms the peeled outer skin and speeds up metabolism. Therefore, it is one of the most important cosmetics compositic which keeps the function of skin normal. When the papain is used in cosmetics with surfactant, the activity of papain is reduced rapidly. In this study, the modified papain with extreme stability negative ionic environment was developed by directed evolution.
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KEYWORD
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Cystein protease, papain, error prone PCR, mutagenesis, staggered extension process, toiletry dopes enzyme
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